Possibility of novel therapeutic strategy for multidrug resistant Pseudomonas aeruginosa using bactericidal activity in Streptococcus sanguinis secretion

Multidrug-resistant Pseudomonas aeruginosa (MDRP) infection has become a serious clinical problem in the currently. We reported the bactericidal activity in the filtrated supernatant of Streptococcus (S.) sanguinis against P. aeruginosa. The S. sanguinis, isolated from the sputum of a pulmonary-disease patient, was cultured for overnight, and filtered supernatant was tested for bactericidal effect using the minimum bactericidal concentration method. The viable number of P. aeruginosa was decreased with time after exposing to the filtrated supernatant of S. sanguinis, and collapsed bacteria were detected with electron microscopy. P. aeruginosa were affected by bactericidal effect. The bactericidal activity was observed in the filtrated supernatant of S. sanguinis against MDRP. The high prevalence of MDRP requires discover pharmaceutical candidates. This new discovery phenomenon may contribute to the development of a novel therapeutic drug against P. aeruginosa.